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rabbit anti c4  (Proteintech)


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    Structured Review

    Proteintech rabbit anti c4
    Rabbit Anti C4, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+c4/C4+alpha+chain%2FC4b%2FC4d+Antibody/pm41168320-80-62-65
    Average 93 stars, based on 17 article reviews
    rabbit anti c4 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Saline:

    Article Title: Rutin ameliorates sevoflurane-induced neurotoxicity by inhibiting microglial synaptic phagocytosis through the complement pathway.
    Article Snippet: .. After being blocked with 5% nonfat milk (dissolved in Tris-buffered saline-Tween) for 2 h at room temperature, the membranes were washed and incubated with the following primary antibodies overnight at 4 °C: rabbit anti-PSD95 (Postsynaptic density protein-95, PSD95)(1:500; Cell Signaling Technology, 2507), rabbit anti-Vglut2 (Vesicular glutamate transporter 2, Vglut2)(1:1000; Synaptic Systems, 135402), rabbit antiC1qa (1:1000; Invitrogen, PA5-29586), rabbit anti-C3 (1:1000; Proteintech, 21337-1-AP), rabbit anti-C4 (1:2000; Proteintech, 22233-1-AP), mouse anti-C5 (1:1500; Proteintech, 66634-1-Ig), and mouse anti-β-tubulin (1:2000; Absin, abs830032). .. The membranes were further incubated with horseradish peroxidase-labeled secondary antibodies: goat anti-mouse (1:1000; Beyotime, A0216) and goat anti-rabbit (1:2000; Beyotime, A0208) for 2 h at room temperature, and the protein expression was detected by Tanon 5200 (Tanon Science & Technology Co. Ltd.).

    Article Title: Rutin ameliorates sevoflurane-induced neurotoxicity by inhibiting microglial synaptic phagocytosis through the complement pathway
    Article Snippet: .. After being blocked with 5% nonfat milk (dissolved in Tris-buffered saline-Tween) for 2 h at room temperature, the membranes were washed and incubated with the following primary antibodies overnight at 4 °C: rabbit anti-PSD95 (Postsynaptic density protein-95, PSD95)(1:500; Cell Signaling Technology, 2507), rabbit anti-Vglut2 (Vesicular glutamate transporter 2, Vglut2)(1:1000; Synaptic Systems, 135402), rabbit anti-C1qa (1:1000; Invitrogen, PA5-29586), rabbit anti-C3 (1:1000; Proteintech, 21337-1-AP), rabbit anti-C4 (1:2000; Proteintech, 22233-1-AP), mouse anti-C5 (1:1500; Proteintech, 66634-1-Ig), and mouse anti-β-tubulin (1:2000; Absin, abs830032). .. The membranes were further incubated with horseradish peroxidase-labeled secondary antibodies: goat anti-mouse (1:1000; Beyotime, A0216) and goat anti-rabbit (1:2000; Beyotime, A0208) for 2 h at room temperature, and the protein expression was detected by Tanon 5200 (Tanon Science & Technology Co. Ltd.).

    Incubation:

    Article Title: Rutin ameliorates sevoflurane-induced neurotoxicity by inhibiting microglial synaptic phagocytosis through the complement pathway.
    Article Snippet: .. After being blocked with 5% nonfat milk (dissolved in Tris-buffered saline-Tween) for 2 h at room temperature, the membranes were washed and incubated with the following primary antibodies overnight at 4 °C: rabbit anti-PSD95 (Postsynaptic density protein-95, PSD95)(1:500; Cell Signaling Technology, 2507), rabbit anti-Vglut2 (Vesicular glutamate transporter 2, Vglut2)(1:1000; Synaptic Systems, 135402), rabbit antiC1qa (1:1000; Invitrogen, PA5-29586), rabbit anti-C3 (1:1000; Proteintech, 21337-1-AP), rabbit anti-C4 (1:2000; Proteintech, 22233-1-AP), mouse anti-C5 (1:1500; Proteintech, 66634-1-Ig), and mouse anti-β-tubulin (1:2000; Absin, abs830032). .. The membranes were further incubated with horseradish peroxidase-labeled secondary antibodies: goat anti-mouse (1:1000; Beyotime, A0216) and goat anti-rabbit (1:2000; Beyotime, A0208) for 2 h at room temperature, and the protein expression was detected by Tanon 5200 (Tanon Science & Technology Co. Ltd.).

    Article Title: Rutin ameliorates sevoflurane-induced neurotoxicity by inhibiting microglial synaptic phagocytosis through the complement pathway
    Article Snippet: .. After being blocked with 5% nonfat milk (dissolved in Tris-buffered saline-Tween) for 2 h at room temperature, the membranes were washed and incubated with the following primary antibodies overnight at 4 °C: rabbit anti-PSD95 (Postsynaptic density protein-95, PSD95)(1:500; Cell Signaling Technology, 2507), rabbit anti-Vglut2 (Vesicular glutamate transporter 2, Vglut2)(1:1000; Synaptic Systems, 135402), rabbit anti-C1qa (1:1000; Invitrogen, PA5-29586), rabbit anti-C3 (1:1000; Proteintech, 21337-1-AP), rabbit anti-C4 (1:2000; Proteintech, 22233-1-AP), mouse anti-C5 (1:1500; Proteintech, 66634-1-Ig), and mouse anti-β-tubulin (1:2000; Absin, abs830032). .. The membranes were further incubated with horseradish peroxidase-labeled secondary antibodies: goat anti-mouse (1:1000; Beyotime, A0216) and goat anti-rabbit (1:2000; Beyotime, A0208) for 2 h at room temperature, and the protein expression was detected by Tanon 5200 (Tanon Science & Technology Co. Ltd.).



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